期刊论文

Zhang, M.

中文

中华危重病急救医学

2095-4352

2017

29

8

684-688

10.3760/cma.j.issn.2095-4352.2017.08.003

国家自然科学基金（81201096,81503445） 湖南省自然科学基金（2016JJ2095）

本校为通讯机构

目的探讨髓样分化因子88(MyD88)和β-干扰素TIR结构域衔接蛋白(TRIF)在脓毒症所致心肌功能障碍中的作用;分析应变率(SR)能否早期敏感评价脓毒症性心功能不全。方法按随机数字表法将64只健康雄性C57BL/6小鼠分为假手术(Sham)组、盲肠结扎穿孔术(CLP)致脓毒症模型组、抗MyD88组和抗TRIF组,每组16只。抗MyD88组和抗TRIF组分别于术前2 h经尾静脉注射抗MyD88抗体5 μL/g或抗TRIF抗体5 μL/g。每组8只动物用于观察24 h存活情况;另8只取心肌组织备检。于术前及术后6 h和12 h用超声心动图评价心功能;术后24 h用聚合酶链反应(PCR)测定心肌组织MyD88、TRIF和炎性因子的mRNA表达,通过检测髓过氧化物酶(MPO)...

Objective To investigate the roles of myeloid differentiation factor 88 (MyD88) and TIR domaincontaining adaptor inducing interferon-β (TRIF) in sepsis-induced myocardial dysfunction, and to analyze whether strain rate (SR) can be early sensitive evaluation for septic heart failure. Methods Sixty-four healthy male C57BL/6 mice were divided into four groups by random number table (n = 16 in each group): sham group, cecum ligation and puncture (CLP)-induced sepsis model group, anti-MyD88 group and anti-TRIF group. The anti-MyD88 group and anti-T...