期刊论文

Effect of microenvirment of heart-blood stagnation syndrome on proliferation of bone marrow mesenchymal stem cells differentiating into cardiomyogenic cells

中文

湖南中医药大学学报

1674-070X

2010

30

9

38-42,53

10.3969/j.issn.1674-070X.2010.09.011.038.05

30772696,30973717:国家自然科学基金 CX2009B168:湖南省研究生创新项目

本校为第一机构

目的 现察心血瘀阻证微环境(Heart-Blood Stagnation Svndrome Microenvirment)对大鼠骨髓间充质干细胞(mesenchymal stem cells,MSCs)向心肌样细胞分化的影响.方法 采用全骨髓细胞体外培养技术分离大鼠MSCs,以流式细胞仪检测细胞表面标志;细胞培养基中加入心血瘀阻证大鼠坏死心肌组织和血清的方法 模拟心血瘀阻证微环境;四甲基偶氮唑盐(MTF)法观察心血瘀阻证微环境对MSCs增值率从而选择最大有效浓度;第4代MSCs进行体外心肌细胞诱导分化;相差显微镜、免疫组化及逆转录聚合酶链反应(RT-PCR)结果 鉴定心肌细胞.结果 大鼠MSCs细胞表面抗原CD11b和CD45阳性细胞率小于2%,CD90阳性率达到98%以上;疫细胞...

Objective To observe the in-vitro effect of microenvirment of heart-blood stagnation syndrome on bone marrow mesenchymal stem cells（MSCs） proliferation and on inducing MSCs to differentiate into cardiomyogenic cells.Methods MSCs were isolated from the cultured all bone marrow cell from adult SD rats and the cell surface marks of MSCs were detected with flow cytometer;the microenvirment was analogue with adding the myocardium lough and serum into the cell culture;the effect of microenvirment on MSCs proliferation was observed with methyl thiaz...