期刊论文

Guo, J.-S.

中文

西安交通大学学报(医学版)

1671-8259

2017

38

4

596-601

10.7652/jdyxb201704026

湖南省高校创新平台开发基金项目（No.11K048）； 贵州省科学技术基金计划项目[No.黔科合基础（2016）1016]； 贵阳中医学院博士基金项目~~；

本校为通讯机构

目的用硝酸甘油型偏头痛大鼠模型,观察芎麻汤药效部位(乙酸乙酯及正丁醇提取部位)对偏头痛发作时三叉神经颈髓复合体(TCC)内降钙素基因相关肽-降钙素受体样受体-活性修饰蛋白受体1(CGRP-CRLR/RAMP1)信号通路的干预作用。方法36只雄性SD大鼠随机分为6组(空白对照组,模型组,芎麻汤药效部位低、中、高剂量组,琥珀酸舒马普坦对照组),每组6只。皮下注射硝酸甘油(10 mg/kg)复制偏头痛模型,给药后乙醚麻醉,其中18只(每组3只)心脏灌注后取全脑剥离TCC部位,采用免疫组化染色检测CGRP、CRLR、RAMP1阳性表达;余18只直接取全脑剥离TCC部位并平分两份,一份组织采用qPCR检测CGRP、CRLR、RAMP1 mRNA表达,另一份...

Objective: To explore the effects of bioactive parts of Xiongma Decoction (parts of ethyl acetate and n-butanol extract) on the CGRP-CRLR/RAMP1 signal pathway so as to clarify its therapeutic mechanism on migraine. Methods: We randomly divided 36 male SD rats into 6 groups with 6 in each: blank group, model group, groups of low-, medium- and high-dose Xiongma Decoction bioactive parts, and Sumatriptan group. By giving hypodermic injection of 10 mg/kg nitroglycerin, the migraine rat model was copied; Only 18 rats were found to have positive expr...