期刊论文

The role of MDM2/p53pathway in regulating apoptosis of multiple myeloma RPMI8226cells

Li, H.

中文

西安交通大学学报(医学版)

1671-8259

2020

41

2

197-200

10.7652/jdyxb202002008

:中医内科重大疾病防治 No.ZYNK201607:教育部重点实验室开放基金 No.2018-01:大学生研究性学习和创新性实验计划 No.81874344:国家自然科学基金 No.2018M640755:中国博士后科学基金 No.2019JJ0307:湖南省自然科学基金面上项目

本校为第一且通讯机构

目的 研究MDM2/p53通路对多发性骨髓瘤(MM)细胞凋亡的调控作用及机制.方法 体外培养人MM细胞株(RPMI 8226)并分为实验组(Nutlin-3a)和对照组(药物溶剂),采用CCK-8法检测各组细胞的增殖情况和抑制率,Western blot检测凋亡相关蛋白表达,流式细胞术检测细胞凋亡情况.结果 与对照组相比,24、48、72 h时间点实验组细胞增殖明显降低,抑制率明显增高(P

Objective:To study the regulatory effect and mechanism of MDM2/p53 pathway on apoptosis in multiple myeloma (MM). Methods: RPMI8226 cells were cultured in vitro and divided into Nutlin-3a group, control group and blank group. CCK-8 method was used to detect the cell inhibition rate, Western blot was used to detect the expression of apoptosis-related protein, and flow cytometry was used to detect the apoptosis in the two groups. Results: Compared with that in control group, the inhibition rate of cells in the experimental group increased signifi...