期刊论文

Effects of Zhuangdu Quhan mixture on miR-29a and Wnt pathway in the ankylosing spondylitis model mice

中文

陕西中医

1000-7369

2020

41

8

1038-1041

10.3969/j.issn.1000-7369.2020.08.006

湖南省技术创新引导计划资助项目(201761) 湖南省中医药管理局资助项目(20177B) 中医内科学省部共建教育部重点实验室开放课题(ZYNK201601)。

本校为第一机构

目的:研究壮督驱寒合剂对强直性脊柱炎（AS）模型小鼠miR-29a及Wnt通路的影响,以探讨壮督驱寒合剂防治AS的机制。方法:将50只6～8周Balb/c雌性小鼠随机分为空白组、模型组、西药组、中药低剂量组、中药高剂量组,每组10只。除空白组外,其余各组均采取蛋白聚糖加完全弗式试剂腹腔注射复制AS小鼠模型。造模结束后各组分别进行相应药物灌胃处理,1次/d,连续6周,末次给药后取膝关节滑膜组织。采用实时荧光定量法测定miR-29a、DKK-1及β-catenin的mRNA表达,采用免疫印迹法检测β-catenin、DKK-1蛋白含量。结果:与空白组相比,模型组小鼠滑膜组织中miR-29a表达升高,β-catenin mRNA及蛋白表达升高,DKK-1 ...

Objective:To study the effect of Zhuangdu Quhan mixture(ZQM)on miR-29a and Wnt pathway in ankylosing spondylitis(AS)model mice,and to explore prevention and treatment mechanism of ZQM for AS.Methods:50 Balb/c mice at 6~8 weeks were randomly divided into normal group,western medicine group,model group,and low,high dose ZQM groups,with 10 mice in each group.Except the normal group,proteoglycan and complete Freund’s reagent were used to establish AS model in each group.After modeling,the corresponding drugs were administered to each group,and the...