期刊论文

Research on effect of Taohong Siwu mixture on healing of acute skin wound of rat based on Hippo signaling pathway

中文

山西中医

1000-7156

2022

38

03

57-61

10.20002/j.issn.1000-7156.2022.03.024

湖南省研究生科研创新项目资助（编号：CX20200810）； 湖南省中医药管理局科研计划项目（编号：2021213）；

本校为第一机构

目的:基于Hippo信号通路观察桃红四物合剂对大鼠急性皮肤创面愈合的影响.方法:将SD大鼠随机分成5组:空白组,模型组,桃红四物合剂低、中、高剂量组,每组各6只.除空白组外,建立大鼠急性全层皮肤创面模型,于术后11天取材,观察各组创面愈合情况,采用HE染色分析创面细胞生物学形态,ELISA法检测血清IL-6、TNF-α含量,免疫组化法分析组织中bFGF、TGF-β1表达量,Western blot法检测Hippo通路相关蛋白MST1、YAP表达情况.结果:桃红四物合剂各组创面愈合率及愈合水平明显优于模型组,创面组织更接近正常皮肤组织结构;与空白组比较,其余各组IL-6、TNF-α表达量明显升高(P＜0.05),桃红四物合剂各组表达均低于模...

Objective:To research the effect of Taohong Siwu mixture on healing of acute skin wound of rat based on Hippo signaling pathway.Methods:SD rats were randomly divided into 5 groups:blank group,model group,Taohong Siwu mixture low-,medium-and high-dose group,6 rats in each group.Except the blank group,the model of acute fullthickness skin wound of rat was established.The samples were taken on the 11 th day after operation,and the wound healing was observed in each group.The cellular biological morphology of wound was analyzed by HE staining.The c...