期刊论文

中文

中华中医药杂志

1673-1727

2016

31

10

4206-4210

国家自然科学基金项目（No.81373702,No.81473567,No.81202632） 教育部博士点基金项目（No.20124323120003） 湖南省自然科学基金项目（No.13JJ3097） 湖南省教育厅科研项目（No.14B134,No.15K092）

本校为第一机构

目的:探讨丹龙醒脑方对局灶性脑缺血再灌注大鼠侧脑室室管膜下区(SVZ)神经干细胞(NSCs)增殖与Frizzled3、Dsh1、β-catenin表达的关系。方法:将120只雄性SD大鼠随机分为假手术组、模型组、依达组、丹龙组。后3组用线栓法制备局灶性脑缺血再灌注(MCAO/R)模型,再灌注7d后取缺血侧SVZ脑组织。采用Brdu免疫荧光法检测SVZ区NSCs增殖,RT-qPCR法、Western Blot法分别检测Frizzled3、Dsh1、β-catenin mRNA和蛋白的表达。结果:与假手术组比较,其余各组Brdu阳性细胞数明显增多,Frizzled3、Dsh1、β-catenin蛋白及 mRNA的表达明显升高(P

Objective: To investigate whether the effects of Danlong Xingnao Formula on promoting proliferation of neural stem cells (NSCs) in subventricular zone (SVZ) were related to the expression of Frizzled3, Dsh1 and β-catenin in hippocampus of rat model of cerebral ischemia-reperfusion (MCAO/R). Methods: One hundred and twenty male SD rats were randomly divided into the sham group, MCAO/R (model) group, edaravone group and Danlong Xingnao Formula (DLXNF) group. The rats in all groups except for the sham group were subjected to a middle cerebral art...