期刊论文

Guo, YT

英文

Journal of Orthopaedic Surgery and Research

1749-799X

2023

18

1

1-14

10.1186/s13018-023-04085-w

This work was supported by grants from the General Project of National Natural Science Foundation of China (No. 8217151084), the Natural Science Foundation of Hunan Province (No. 2022JJ70029), the Changsha Natural Science Foundation (No. kq2202460), the Hunan Provincial Health Commission Project (No. 202204074858) and the Scientific Research Project of Hunan Administration of Traditional Chinese Medicine (No. D2022094).

本校为第一且通讯机构

To explore the mechanism of psoralen synergized with exosomes (exos)-loaded SPC25 on nucleus pulposus (NP) cell senescence in intervertebral disc degeneration (IVDD). IVDD cellular models were established on NP cells by tert-butyl hydroperoxide (TBHP) induction, followed by the treatment of psoralen or/and exos from adipose-derived stem cells (ADSCs) transfected with SPC25 overexpression vector (ADSCs-oe-SPC25-Exos). The viability, cell cycle, apoptosis, and senescence of NP cells were examined, accompanied by the expression measurement of aggrecan, COL2A1, Bcl-2, Bax, CDK2, p16, and p21. Afte...