期刊论文

Effects of Chinese herbal extract HNA-1 on the monocyte subsets in SIV chronically infected rhesus macaques

中文

中华中医药杂志

1673-1727

2017

32

12

5543-5547

国家自然科学基金项目(No.81274169,No.81573822)； 湖南省高等学校科学研究重点项目(No.12A102)~~；

本校为第一机构

目的:探讨中药复方提取物湘A-1(HNA-1)对猴免疫缺陷病毒(SIV)慢性感染恒河猴单核细胞亚群的影响,以进一步了解HNA-1促进胸腺输出、提升初始型CD4的机制。方法:采用SIVmac239病毒液对8只中国恒河猴慢性感染16-21个月,随机分为对照组和治疗组,每组4只。治疗组给予HNA-1灌胃治疗,每日1次;对照组给予等体积0.9%氯化钠溶液灌胃。两组给药2个月。在治疗第0、28、56天采集血液检测血常规,并采用流式细胞法检测外周血单核细胞CD14~(++)CD16~-(经典型)、CD14~(++)CD16~+(中间型)和CD14dimCD16~+(非经典型)亚群的比例、各亚群表达HLA-DR、趋化因子CCR5和CCR7的情况,以及外周血单个核细胞(PBMC)表达白细胞介...

Objective: To observe the effects of Chinese herbal extract HNA-1 on the proportion of monocyte subsets in SIV chronically infected monkeys, thereby to understand the underlying mechanisms of HNA-1 boosting thymic output and increasing peripheral naive CD4 cells. Methods: Eight Chinese rhesus macaques had been infected by SIVmac239 for 16 to 21 months were enrolled into this study, and assigned randomly to treatment group (receiving HNA-1 administration for consecutively 2 months) or control group. At the 0, 28th, 56th day, blood cell analysis ...