期刊论文

To explore the preventive and therapeutic effects of Xuebijing injection on acute lung injury induced by cardiopulmonary bypass in rats by regulating the expression of microRNA-17-5p and its mechanism

中文

中华危重病急救医学

2095-4352

2019

31

7

867-872

10.3760/cma.j.issn.2095-4352.2019.07.014

湖南省中医药科研计划项目(201696) 中医内科学省部共建教育部重点实验室开放基金(ZYNK201506) 湖南中医药大学中医学国内一流建设学科项目(2018-469).

本校为第一机构

目的探讨血必净注射液对体外循环(CPB)致急性肺损伤(ALI)的防治作用及机制。方法①体内实验:将30只成年雄性SD大鼠按随机数字表法分为假手术组(Sham组)、CPB组、血必净预处理组(XBJ+CPB组),每组10只。建立大鼠CPB模型;Sham组仅经动静脉穿刺,不进行CPB。XBJ+CPB组在CPB前2 h腹腔注射4 mL/kg血必净注射液;Sham组和CPB组给予等量生理盐水。术后4 h取股动脉血进行血气分析,计算氧合指数(PaO_2/FiO_2)。然后处死大鼠收集支气管肺泡灌洗液(BALF),计算肺通透性指数(PPI);取肺组织检测湿/干重比值(W/D),测定肺泡损伤数定量评价指标(IQA),采用酶联免疫吸附试验(ELISA)检测肺组织及BALF中白细胞介素(IL-1、...

Objective To investigate the preventive effect of Xuebijing injection on acute lung injury induced by cardiopulmonary bypass (CPB) and the underlying mechanism. Methods ① In vivo experiment: 30 Sprague-Dawley (SD) rats were randomly divided into sham group, CPB group, Xuebijing pretreatment group (XBJ+CPB group) with 10 rats in each group. CPB model was reproduced in rats; and CPB was not performed in sham group, but only through arteriovenous puncture. In the XBJ+CPB group, 4 mL/kg Xuebijing injection was injected intraperitoneally 2 hours be...