期刊论文

The mechanism of Xuebijing injection in preventing and treating lung injury induced by cardiopulmonary bypass by regulating the apoptosis of alveolar polymorphonuclear neutrophil

中文

中华危重病急救医学

2095-4352

2024

36

2

166-171

10.3760/cma.j.cn121430-20230128-00038

本校为第一机构

目的:从调控中性粒细胞（PMN）凋亡探讨血必净注射液对体外循环（CPB）所致急性肺损伤（ALI）的防治作用。方法:按随机数字表法将30只雄性SD大鼠分为假手术组（Sham组）、CPB模型组（CPB组）、血必净预处理组（XBJ组），每组10只。CPB组和XBJ组CPB 60 min后停机；Sham组不行CPB。XBJ组CPB前2 h腹腔注射4 mL/kg血必净注射液；Sham组和CPB组注射等量生理盐水。CPB后4 h，取动脉血进行血气分析，计算呼吸指数（RI）；取肺组织测定肺系数（LI）和肺含水率。收集支气管肺泡灌洗液（BALF）获取PMN，检测天冬氨酸特异性半胱氨酸蛋白酶3（caspase-3）活性，并用流式细胞仪检测细胞凋亡率；实时荧光定量聚...

Objective:To investigate the protective effect of Xuebijing injection on acute lung injury (ALI) associated with cardiopulmonary bypass (CPB) by regulating the apoptosis of polymorphonuclear neutrophils (PMN).Methods:Thirty male Sprague-Dawley (SD) rats were randomly divided into sham operation group (Sham group), CPB model group (CPB group) and Xuebijing pretreatment group (XBJ group) according to the random number table method, with 10 rats in each group. Rats in the CPB group and XBJ group undergoing CPB procedures for 60 minutes. Rats in th...