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An end-point method based on graphene oxide for RNase H analysis and inhibitors screening

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成果类型:
期刊论文
作者:
Zhao, Chuan;Fan, Jialong;Peng, Lan;Zhao, Lijian;Tong, Chunyi*;...
通讯作者:
Tong, Chunyi;Liu, Bin
作者机构:
[Liu, Bin; Fan, Jialong; Peng, Lan; Zhao, Chuan; Zhao, Lijian; Tong, Chunyi; Liu, B] Hunan Univ, Coll Biol, Hunan Prov Key Lab Plant Funct Genom & Dev Regula, Changsha 410082, Hunan, Peoples R China.
[Wang, Wei] Hunan Univ Chinese Med, Sch Pharmaceut Sci, Changsha 410208, Hunan, Peoples R China.
通讯机构:
[Tong, CY; Liu, B] H
Hunan Univ, Coll Biol, Hunan Prov Key Lab Plant Funct Genom & Dev Regula, Changsha 410082, Hunan, Peoples R China.
语种:
英文
关键词:
Graphene Oxide;RNase H;Inhibitor screening;HBV
期刊:
Biosensors and Bioelectronics
ISSN:
0956-5663
年:
2017
卷:
90
页码:
103-109
基金类别:
This work was partially supported by the Natural Science Foundation of Hunan Province ( h14JJ2049 ), the Science Foundation for Outstanding Young Scholars of Hunan Province ( 2015JJ1007 ), the Natural Science Foundation of China for Young Scholars ( 31201074 ) andthe Fundamental Research Funds for the Central Universities of China ( 2015JCA03 ). The authors thanks Prof. Xuanming Liu of Hunan University for providing experimental conditions and suggestive advice and Dr. Michael Mauk from University of Pennsylvania in polishing the language of the manuscript.
机构署名:
本校为其他机构
院系归属:
药学院
摘要:
As a highly conserved damage repair protein, RNase H can hydrolysis DNA-RNA heteroduplex endonucleolytically and cleave RNA-DNA junctions as well. In this study, we have developed an accurate and sensitive RNase H assay based on fluorophore-labeled chimeric substrate hydrolysis and the differential affinity of graphene oxide on RNA strand with different length. This end-point measurement method can detect RNase H in a range of 0.01 to 1 units /mL with a detection limit of 5.0×10-3units/ mL under optimal conditions. We demonstrate the utility of the assay by screening antibiotics, resulti...

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