期刊论文

PA-MSHA Inhibits Growth of Human Cervical Cancer Cell Line Hela via a Mannose dependent Manner

中文

实用预防医学

1006-3110

2013

20

9

1040-1044

10.3969/j.issn.1006-3110.2013.09.006

湖南省科技厅计划项目（2013SK3216）； 湖南省第二人民医院重点专科基金；

本校为第一机构

目的 研究铜绿假单胞菌制剂(pseudomonas aeruginosa with mannose sensitive hemagglutination pili,PAMSHA)由甘露糖依赖方式影响Bad、Bax及Bcl-2的表达进而影响癌细胞的增殖和细胞周期进程,为治疗宫颈癌的新的生物制剂提供依据. 方法 采用单独处理PA-MSHA或甘露糖与PA-MSHA联合处理宫颈癌Hela细胞,随后采用MTT法检测细胞增殖,流式检测细胞周期,荧光定量PCR和western blot检测凋亡抑制蛋白Bcl-2和促凋亡蛋白Bad、Bax的表达变化. 结果 PA-MSHA单独处理宫颈癌Hela后细胞增殖减慢,细胞周期阻滞在G0/G1期,同时荧光定量PCR和western blot检测显示促凋亡蛋白Bad、Bax的表达增加而凋亡抑制蛋白Bcl-2表...

Objective To investigate the effects of Pseudomonas aeruginosa with mannose sensitive hemagglutinin （PA- MSHA） on the proliferation and cell cycle of cervical cancer cell line Hela via a mannose- dependent manner. Methods Hela cells were treated with PA- MSHA or PA MSHA and mannose. The cell proliferation was observed by MTT. Cell cycle distri- bution was measured by flow cytometry （FCM）. Real time RT-PCR and western blot were used to evaluate the expressions of apoptosis - related molecules, Bad, Bax and Bcl - 2. Results For the Hela cells...