期刊论文

中文

湖南中医药大学学报

1674-070X

2016

36

8

17-21

10.3969/j.issn.1674-070X.2016.08.005

81202632、81473567:国家自然科学基金 20124323120003:高等学校博士学科点专项科研基金 13JJ3097:湖南省自然科学基金 14B134、15K092:湖南省教育厅科学研究项目

本校为第一机构

目的：探讨健脾补土法组方含药血清对体外嗅鞘细胞低氧/复氧损伤模型核因子KB（nuclear factor-kappa B,NF-KB）的亚单位P65、P50及NF-KB抑制蛋白（P-IκBα）表达的影响。方法将原代培养的嗅鞘细胞分为5组：正常血清对照组、正常血清+低氧组、低氧+依达拉奉含药血清组、低氧+健脾补土法组方含药血清组、正常血清+低氧+NF-KB抑制剂/抗氧化剂（PDTC，50μmol/L）组，采用微需氧袋低氧/复氧的方法诱导嗅鞘细胞形成脑缺血再灌注损伤样模型，用Western blot法检测嗅鞘细胞胞浆P65、P50、P-IκBα和胞核P65、P50的含量。结果与正常血清对照组比较，正常血清+低氧组胞浆和胞核P65、P50，胞浆P-IκBα表...

Objective To investigate the effect of spleen-strengthening therapy contained serum on subgene P65, P50 of nudear factor-kappa B (NT-KB) and P-IκBα expression in olfactory ensheathing cells with hypoxia/reoxygenation injury. Methods Primary cultured olfactory ensheathing cells were randomly divided into five groups: normal serum control group, normal serum+hypoxia group, hypoxia+edaravone contained serum group, hypoxia+spleen-strengthening therapy contained serum group, normal serum+hypoxia+NF-KB inhibitor/antioxidant (PDTC 50 μmol/L) group....