期刊论文

Guo Zhi-Hua<&wdkj&>Li Ya

中文

数字中医药(英文)

2096-479X

2019

2

4

237-256

10.1016/j.dcmed.2020.01.005

funding support from the National Natural Science Foundation of China （No. 81673955）； the Education Department of Hunan Province （No. 16A158）； Domestic First-class Construction Discipline Open Fund Project for Chinese Medicine of Hunan （No. 2018ZYX43）； the Postgraduate Innovation Project of Hunan University of Chinese Medicine in 2018 （No. 2018CX43）；

本校为第一且通讯机构

目的 应用网络药理学方法探讨益心泰方治疗心力衰竭潜在的作用机制,并通过Western blotting实验对相关靶点作初步验证.方法 以心力衰竭为疾病模型,应用TCMSP数据库及其分析平台,根据OB和DL筛选益心泰方的活性成分,并预测出相应靶标;经HPO数据库检索心力衰竭的疾病靶标;然后在STRING数据库构建"益心泰成分-潜在靶标-心力衰竭"网络,通过Cytoscape软件将网络可视化,再根据D,CC和BC得到网络的关键节点,借助GATHER数据库平台对关键节点进行KEGG的基因功能富集分析.用缩窄腹主动脉法 + PTU 灌胃建立心衰兔模型,通过Western blotting法观察兔心肌组织TGF-β蛋白的病理改变及蛋白表达情况.结果 "益心泰方...

Objective: To explore the possible mechanism of Yi Xin Tai Formula (YXTF) in treating heart failure (HF) by network pharmacology method and to verify the related targets by western blotting. Methods: Using the traditional Chinese medicine systems pharmacology (TCMSP) database with its analysis platform for HF as a disease model, the active ingredients of YXTF were filtered according to the oral bioavailability (OB) and drug- likeness (DL), and the corresponding targets were predicted. The disease targets of HF were retrieved by Human Phenotype ...