期刊论文

中文

神经解剖学杂志

1000-7547

2014

30

5

581-586

10.11670/1000-7547.201405014

本校为其他机构

目的:探讨脑内注射内毒素脂多糖(lipoolysaccharide,LPS)诱导的大鼠慢性神经炎症脑内β-位点淀粉蛋白剪切酶-1(β-site amyloid precursor protein cleavage enzyme-1,BACE-1)的表达变化。方法:依赖立体定位技术,在成年SD雄性大鼠右侧海马内注入LPS,动物存活15 d。运用免疫组织化学方法检测大鼠脑内BACE-1,β-淀粉样前体蛋白(β-amyloid precursor protein,APP)以及β-淀粉样蛋白(β-amyloid,Aβ)等阿尔茨海默病(Alzheimer's disease,AD)相关蛋白的表达变化;采用免疫荧光双重标记技术,观察BACE-1在大鼠脑内的表达情况和定位分布。结果:在正常哺乳动物的大脑内,BACE-1主要表达在海马苔藓纤维和嗅...

Objective: To investigate the expression changes of β-site amyloid precursor protein cleavage enzyme-1(BACE-1)after chronic brain inflammatory induced by lipopolysaccharide(LPS). Methods: Depending on three-dimensional positioning technology,we injected LPS to the right lateral hippocampus of adult male SD rats. Animals were survived for 15 days. Immunohistochemistry was used to detect the expression of BACE-1 and Alzheimer's disease immune related molecules such as the APP and Aβ in the cerebral cortex and hippocampus. The expression positio...