期刊论文

An investigation on the proteomics of nasopharyngeal carcinoma cell line HNE1 treated with Qi-boosting toxin-resolving granule

中文

中国中西医结合耳鼻咽喉科杂志

1007-4856

2004

12

3

120-123

10.16542/j.cnki.issn.1007-4856.2004.03.004

39770933,301711 74,30271678:国家自然科学基金 95B063,02-03JP29:国家中医药局科研项目 01A014:湖南省教育厅科学研究项目

本校为其他机构

目的探讨中药复方益气解毒颗粒对鼻咽癌细胞杀伤作用的分子机制.方法筛选益气解毒颗粒药液对鼻咽癌杀伤的半抑制浓度(IC50),用0.1倍半抑制浓度(0.1×IC50)的益气解毒颗粒作用鼻咽癌细胞HNEl 96h,用固相pH梯度双向凝胶电泳分离益气解毒颗粒处理和未处理的鼻咽癌细胞HNE1的总蛋白质,银染显色,PDQuest 2-de软件分析差异蛋白质点.结果益气解毒颗粒药液杀伤鼻咽癌细胞HNE1的半抑制浓度为6.25mg/ml;双向电泳图像分析益气解毒颗粒药物处理组图像的平均蛋白质数为1120±89,未处理组的平均蛋白质数为1281±102;差异蛋白质有673个,其中有218个表达增强,有455个蛋白质表达下降.结论益气解毒颗粒对鼻咽癌细胞...

Objective To investigate the molecular mochanism of Qi-Boosting Toxin Resolving Granule (QBTRG)in the inhibition of nasopharyngeal carcinoma cell line HNE1 based on the techniques of proteomics. Methods The IC_(50) of QBTRG killing HNE1 cells was determined by MTT at first. Then, HNE1 cells were treated with QBTRG at the concentration of 0.1×IC_(50) for 96 hours. After this step, the cells were collected and the total protein was extracted from these cells. By two-dimension clec- trophoresis, the proteins were separated and protein profiles we...