期刊论文

The Features of Gene Expression Profile on A Area of AtlasTM cDNA Array among Rats with Qi Deficient Syndrome

中文

湖南中医药大学学报

1674-070X

2001

21

2

1-3

10.3969/j.issn.1674-070X.2001.02.001

国家自然科学基金! （39770 933）； 国家中医药管理局基金! （95B0 6 3）； 中南大学***基金；

本校为第一机构

探讨气虚证模型大鼠A区基因表达谱特征及其对治疗的反应。采用药物加疲劳法制造Wistar大鼠气虚模型，AtlasTM反义DNA(cDNA)阵列法检测造模动物鼻咽上皮细胞基因组A区基因表达谱。结果：模型组动物表现细胞核转录因子亚单位基因c-jun和c-kit原癌基因表达下调，前胸腺素α和钙结合蛋白2基因表达上调。治疗1周后，表达上调的2基因活性恢复正常，但表达下调的2基因活性仍维持低表达状态。结论：单纯气虚证动物出现原癌基因和应激反应相关基因表达活性异常，其变化趋势和病理生理意义在于尽力维持机体内环境的稳定，即阴阳平衡。

To investigate the features of gene expression profile on A area of Atlas TM cDNA array and their response to treatment among rats with qi deficient syndrome.Qi deficient syndrome was induced in rats with the method of herbal decoction perfused gastrically and supplemented by forced swimming. The expressive features of genes on A area were determined in nasopharyngeal tissues of tested rats on Atlas TM cDNA expression array.Results: The expressive activities of c jun and c kit proto oncogenes were downwards regulated,and those of prothymosin α...