期刊论文

Effect of Bu Yang Huan Wu Decoction and Its Available Composition Combination on Reactive Astrocyte Activation Following Cerebral Ischemia in Gerbils

中文

湖南中医药大学学报

1674-070X

2001

21

4

5-8

10.3969/j.issn.1674-070X.2001.04.002

国家自然科学基金资助项目 （3 9770 893 ）；

本校为第一机构

目的比较研究补阳还五汤及其有效部位组方对脑缺血后反应性星形胶质细胞活化的影响.方法采用沙土鼠双侧颈总动脉夹闭脑缺血模型,分别腹腔注射补阳还五汤及其有效部位组方,于不同时间取脑组织,以免疫组化方法测定胶质纤维酸性蛋白(GFAP)的表达.结果缺血15?min后各组脑组织无GFAP表达.缺血15?min再灌注24?h后,脑组织GFAP表达达高峰,主要在海马区表达,补阳还五汤及其有效部位组方可使GFAP表达减弱,两者作用相近.缺血15?min再灌注48?h后,GFAP表达有所减弱,而补阳还五汤及其有效部位方组GFAP表达则较模型组有所增强,两者作用相近.结论星形胶质细胞活化主要在脑缺血后再灌注期,在再灌注早期,补阳还五汤...

Objective: To compare the effects of Bu Yang Huan Wu Decoction(BYHWD) and its available composition combination on reactive astrocyte activation following cerebral ischemia and reperfusion in gerbils.Method: The Cerebral ischemia model was made by ligating bilateral common carotid arteries in gerbil. BYHWD and its available composition combination were administered intraperitoneally repectively. Cerebral tissues were taken at different time. Expression of glial fibrillary acidic protein(GFAP) was determined by immunohistochemical method. Results:There was no expression of GFAP in cerebral tiss...