期刊论文

Antagonistic Effect of Naotaifang Extract on the Injury of Human Umbilical Cord Endothelical Cells Induced by Recombined Human Tumor Necrosis Factor Alpha

中文

湖南中医药大学学报

1674-070X

2002

22

4

7-9,23

10.3969/j.issn.1674-070X.2002.04.003

湖南省自然科学基金资助项目 （98JJY2 0 2 7）；

本校为第一机构

目的观察肿瘤坏死因子α(TNF-α)对培养的人脐静脉内皮细胞的损伤作用及中药脑泰方提取物(NTE)的保护作用.方法将融合生长人脐静脉内皮细胞(HUEC)随机分为空白对照组、TNF-α组、NTE对照组和NTE高、中、低剂量组,观察100?ng/ml的TNF-α作用24?h后内皮细胞显微形态的改变及培养液中血管性假血友病因子(vWF)含量.结果 TNF-α作用后细胞形态发生显著变化,相差显微镜及普通光镜下主要表现为较多细胞收缩成圆形固缩状,细胞间隙变宽,甚至发生脱落并出现异常颗粒;部分细胞纵轴拉长,横轴缩小,表现为成纤维细胞型外观,培养液中vWF含量显著升高(P

?Objective To observe the injury effect on human umbilical cord endothelial cells (HUECs) induced by recombined human trmor necrosis factor alpha (rhTNF α) and the antagonistic effect of Naotaifang Extract(NTE)on it.Methods The cultured HUECs were randomly divided into five groups,i.e.controlling group,rhTNF α group(100?ng/ml rhTNF α),3 NTE groups with different dosages (100?ng/ml of rhTNF α and 6?mg/ml,2?mg/ml or 0.67?mg/ml of NTE).Morphologic changes of HUECs and von willebrand factor(vWF)contents in the conditioned media were detected af...