期刊论文

Antagonistic Effect of Naotaifang Extract on the Apoptosis of Human Umbilical Cord Endothelial Cell Induced by rhTNF-α

中文

湖南中医药大学学报

1674-070X

2003

23

4

1-3

10.3969/j.issn.1674-070X.2003.04.001

湖南省中青年科技基金资助项目 （ 0 0JZY2 14 5 ）； 湖南省自然科学基金资助项目 （ 98JJY2 0 2 7）；

本校为第一机构

目的观察重组人肿瘤坏死因子α(rhTNF-α)诱导人脐静脉内皮细胞(HUEC)凋亡及中药脑泰方提取物(NTE)的作用.方法将融合生长的HUEC随机分为空白对照组(正常培养,不加rhTNF-α)、NTE对照组(加中剂量NTE,不加rhTNF-α)、rhTNF-α组(仅加100 ng/mL rhTNF-α)和NTE高、中、低剂量组(加100ng/mLrhTNF-α和6 mg/mL、2mg/mL、0.67mg/mLNTE),观察rhTNF-α作用24h后内皮细胞吖啶橙/溴乙啶双重荧光染色和凋亡细胞计数.结果荧光显微镜下可见,空白对照组和NTE对照组HUEC为正常结构的活细胞、染色质主要着亮绿色,凋亡细胞率分别为14.0%和14.5%;rhTNF-α组与NTE低剂量组细胞大多呈均匀橙色,染色质多被染为橘红色...

?Objective To observe the apoptosis inducing effect of recombined human tumor necrosis factor alpha(rhTNF α) on human umbilical cord endothelial cells(HUCEs) and the antagonistic effect of Naotaifang extract(NTE) on it. Methods The cultured HUCEs were randomly divided into 6 groups,including blank controlling group (BCG),NTE controlling group(NCG)with mid dose NTE added only,rhTNF α group(TNG) with 100?ng?/ml rhTNF α added only and 3 treating groups(TG1～3) with various dosage of NTE(100?ng rhTNF α plus 6?mg?/ml,2?mg?/ml or 0.67?mg?/ml NTE)...