期刊论文

中文

湖南中医药大学学报

1674-070X

2004

24

4

20-22

10.3969/j.issn.1674-070X.2004.04.008

湖南省中医药管理局基金项目 （ 2 0 0 0 0 2 5 ）；

本校为第一机构

目的建立春雨烧伤膏中盐酸小檗碱含量的高效液相色谱法.方法色谱柱为Hypersil ODS C18 5μmφ4.6×100mm;流动相为乙腈-1%磷酸溶液-三乙胺(25:75:0.75),检测波长为345 nm;柱温为45℃;流0速为1.0mL/min.结果盐酸小檗碱在57.5～575ng(r=0.999 5,n=6)范围内呈良好的线性关系;平均回收率为99.23%,RSD为1.40%.结论用高效液相色谱法检测简便、快速,定量准确,可用于春雨烧伤膏的定量分析.

Objective To establish a measuring method for content determination of berberine hydrochloride in Chunyu Burn Ointment by HPLC.Methods The content of berberine was determined by HPLC under the conditions with stationary phase being Hypersil ODS C 18 (4.6×100mm, 5μm),mobile phase being Acetonitrile-1% H 3PO 4-Triethylamine(25∶75∶0.75),detecting wavelength being 345nm, flowing rate being 1.0ml/min and column ternperature being 45℃.Results There was a good linear correlation in the range of 57.5 ～575.0ng (r=0.9995,n=6) with the content of ...