期刊论文

A Study on the Content of Coffee Acide in Honeysuckle's Main Variety, L. Macrathoides Hands Mazz. in Hunan.

中文

湖南中医药大学学报

1674-070X

2005

25

4

13-14

10.3969/j.issn.1674-070X.2005.04.005

湖南省科技厅“十五”重大项目:湖南省大宗道地药材种质资源的研究（01C236）。；

本校为第一机构

目的采用HPLC测定湖南金银花灰毡毛忍冬花蕾中咖啡酸的含量.方法采用C18柱;乙晴:0.4%磷酸(11:87)为流动相;检测波长327 nm.结果咖啡酸的平均回收率为97.82%(RSD=1.49%,n=3).结论HPLC测定忍冬花中咖啡酸方法简单,准确,适应于灰毡毛忍冬原药材及其提取物的质量研究.

Objective To determine the content of coffee acide in Lonicera macrathoides Hands Mazz. by HPLC. Methods ODS was C18 ; The flowing condition was acetonitrile： 0.4 % H3PO4 （ 11 ： 87） ;λ = 327nm. Results The coffee acide＇ s RSD was 1.49%, n = 3. Conclusion The method is simple and accurate, fitting quality studies on the original medicinal and its ex...