期刊论文

Study on browning control in callus induced by stem fragments of Maganolia biloba （Rehd et Wils）cheng

中文

湖南中医药大学学报

1674-070X

2007

27

4

36-37

10.3969/j.issn.1674-070X.2007.04.013

湖南省教育厅科研资助项目（03C292）；

本校为第一机构

目的 研究愈伤组织诱导过程中的褐变问题.方法 :将采集的标本进行外植体灭菌法再接种到培养基,在培养基中加抗褐变荆,进行防褐变试验.结果 外植体在4℃冰箱冷藏48 h的预处理对褐变有较好的控制效果.在培养基中加入抗褐变剂比加入吸附剂抗褐变效果好,抗褐变剂中Vc的效果最佳,吸附荆PVP比活性发好.结论 培养条件,以低温遮光处理效果较好,先、温两个因素中,凹叶厚朴对光敏感,遮光能有效抑制褐变.液体浅层静置培养10 d后转入固体培养抗褐变的效果比单纯的固体培养效果好.

Objective To study the brown staining of Maganolia biloba （Rehd et Wils）cheng stem fragments as explants during inducing the callus. Method The explant was sterilized and inoculated in a culture medium in which the anti-brown staining agent was added. Results The pretreated explants in 4 ~C for 48 h were effective to control brown staining; The anti-browning agent added into the medium were better than the adsorbents into medium; Vitamin C was the best material in all anti-browning stain agents, and the adsorbent PVP was better than active ca...