期刊论文

中文

湖南中医药大学学报

1674-070X

2007

27

2

12-14

10.3969/j.issn.1674-070X.2007.02.005

国家“十五”科技攻关课题（2004BA721A27）；

本校为第一机构

目的 建立一种适合RAPD分析的百合鳞叶总DNA提取方法.方法 在传统CTAB提取方法上,对其进行改良,进一步优化提取方法.结果 用改良CTAB法提取的总DNA的OD260/OD280瑚在1.6-2.0之间,电泳条带清晰,其含量和纯度完全满足RAPD分析的要求.结论 该方法提取百合鳞叶总DNA简便实用.

Objective To excogitate a method for extracting DNA from Bulbus Lilii to be appropriate for RAPD analysis. Methods Based the traditional CTAB, the method was improved to obtain an extrcting method. Results The OD260/OD280 of total DNA was between 1.6 to 2.0 with the improved method of CTAB, the banding patterns was clear, and the content and pureness of the total DNA can meet the reaction for RAPD. Conclusions A simple and useful method for DNA...