期刊论文

Study on supercritical CO2 fluid extraction of five drugs in formula Xuanyunding

中文

湖南中医药大学学报

1674-070X

2009

29

4

41-43

10.3969/j.issn.1674-070X.2009.04.013

湖南省教育厅科研基金资助项目（01C235）； 湖南省卫生厅科研基金资助项目（C2005-045）；

本校为第一机构

目的 优选超临界CO2萃取眩晕定方中5味药材的工艺条件.方法 以萃取率、丹参酮ⅡA、阿魏酸、丹皮酚含量为评价指标,高效液相色谱法定量,采用正交设计试验法,探讨超临界CO2萃取眩晕定方中5味药材的萃取温度(A)、萃取压力(B)、夹带剂(C)、萃取时间(D)等最佳工艺参数.结果 超临界最佳提取工艺条件为萃取温度40℃、萃取压力25 MPa、夹带荆95%乙醇、萃取时间1.5 h.结论 该提取工艺有效成分损失少,生产周期短,工艺稳定可行,适合于药厂加工生产.

Objective To optimize the conditions of supercritical fluid extraction for Xuanyunding. Methods To take the extraction rate, tanshinone Ⅱ A, ferulic acid and paeonol as the evaluation indexes, the optimal process parameters of extraction temperature （A）, extraction pressure （B）,co-solvent （C）and extraction time （D）were studied with orthogonal test and HPLC. Results The conditions of superior SFE-CO2 technology were extracting temperature 40℃ ,extracting time 1.5 h,extracting Pressure 25 Mpa and co-solvent was 95%et hanol. Conclusion T...