期刊论文

Effect of Wuhu decoction on dendritic cells stimulating T-lymphocytes to secret IL-4 and IL-5 in asthma infants

中文

湖南中医药大学学报

1674-070X

2009

29

2

49-51

10.3969/j.issn.1674-070X.2009.02.017

国家自然科学基金资助项目（30572406）；

本校为第一机构

目的 观察五虎汤对哮喘婴幼儿DC刺激T淋巴细胞分泌IL-4、IL-5的影响.方法 将60例哮喘婴幼儿随机分为五虎汤治疗组和西药对照组(分别简称治疗组,对照组)各30例,另选健康婴幼儿10例.以Thomas法分离、培养并诱导治疗组及对照组哮喘婴幼儿和健康婴幼儿外周血DC细胞,并与健康儿童的T淋巴细胞共同培养后,采用酶联免疫双抗体夹心法(ELISA)检测各组患儿血清中IL-4、IL-5浓度.结果 婴幼儿哮喘患儿DC刺激T淋巴细胞分泌IL-4、IL-5较健康组显著升高,差异具有统计学意义(P＜0.01);五虎汤治疗后IL-4、IL-5均较治疗前显著降低,差异具有统计学意义(P＜0.05或P＜0.01),且治疗组下降幅度均较对照组显著,差异具有统...

Objective To observe the effect of Wuhu decoction on dendritic cells （DC） stimulating T-lymphocytes to secret interleukin-4（IL-4） and interleukin-5（IL-5） in asthma infant. Methods 60 infants with asthma were randomly divided into two groups; Wuhu decoction treatment group（group A） and Western medicine control group（group B）, and another 10 healthy infants were randomly selected as group C. The DC, from peripheral blood of both asthmatic infants in 3 groups, were separated and cultured fixed the health and asthma cells in vitro with Th...