期刊论文

中文

湖南中医药大学学报

1674-070X

2009

29

2

37-39

10.3969/j.issn.1674-070X.2009.02.013

国家科技部“十一五”科技支撑计划资助项目（2006BA106A11-06） 湖南省中医药管理局资助项目（2008089）.

本校为第一机构

目的 用HPLC法测定不同来源百合中薯蓣皂苷元的含量.方法 采用Phenomenex ODS(4.6 mm×250mm,5 μm)色谱柱,乙腈-水梯度洗脱,流速为0.7 mL/min,进样量为20 μL,检测波长为203nm,柱温为30℃.结果 薯蓣皂苷元的进样量在0.04～1.60μg范围内与峰面积呈良好的线性关系.平均回收率为98.2%,RSD为2.1%(n=6).结论 该法操作简便、准确、灵敏度高,可作为药用百合质量控制的定量方法.

Objective To determine the diosgenin contents in Bulbus lain from different areas with RP-HPLC. Methods The RP-HPLC were with＇4.6 mm×250 mm, 5μm phenomenex ODS column, mobile phase of acetonitrile-water, 0.7 mL/min flow rate, 20 μL sample injection, 203nm wavelength and 30 ℃ temperature. Results The best linear correlation was the sample injection in the range of 0.04-1.60 μg and peak area（n=6）; and the average recoveries of diosgenin was 98.2%（RSD=2.1%,n=6）. Conclusion The methods is simple and accurate with a good sensitivity, whic...