期刊论文

中文

湖南中医药大学学报

1674-070X

2009

29

6

56-59

10.3969/j.issn.1674-070X.2009.06.018

2005CB523306:国家重点基础研究发展计划（973计划） 30901901:国家自然科学基金 06JJ50049:湖南省自然科学基金

本校为第一机构

目的 观察不同强度针刺对心肌缺血大鼠血清IMA及下丘脑、脊髓5-羟色胺(5-HT)表达的影响及作用机制;并分析比较不同强度针刺内关对心肌缺血疗效的差异.方法 将50只SD大鼠随机分为空白组、假手术组、模型组、轻手法针刺组、重手法针刺组.采用冠状动脉结扎法建立心肌缺血再灌注模型,运用721分光光度计测定血清缺血修饰白蛋白含量,免疫组化法测定脊髓背角和背根神经节5-HT阳性表达.结果 模型组大鼠心肌缺血再灌注后心电图ST段电位值、血清IMA含量、5-HT阳性表达均较空白组和假手术组明显升高,差异有统计学意义(P＜0.01),两个针刺组与之比较显著降低,但仍高于空白组和假手术组,差异有统计学意义(P＜0...

Objective To explore the mechanism of acupuncture on protecting MIRI by observing the effects of different acupuncture intensities on IMA in the serum and the expression of 5-HT in the hypothalamus and the spinal cord in rats with MIRI and compare the differences of protective effect by stimulating Neiguan point among different stimulating intensities. Methods 50 SD rats were randomly divided into 5 groups： blank group, sham operation group, model group, weak acupuncture intensity group and strong acupuncture intensity group. The MIRI model wa...