期刊论文

中文

湖南中医药大学学报

1674-070X

2010

30

3

48-50

10.3969/j.issn.1674-070X.2010.03.015.048.03

湖南省中医管理局科研基金资助项目（2006-084）；

本校为第一机构

目的 探讨骨碎补不同溶液制备中柚皮苷含量的最佳测定方法 .方法 供试品溶液分别采用回流3 h与6 h、称质量回流3 h、索氏回流3 h与6 h等5种方法 制备.HPLC法测定含量,以Hypersil C18色谱柱为固定相;甲醇-醋酸-水(40:3:60)为流动相;流速1.0 mL/min;柱温25℃;检测波长283 nm.结果 柚皮苷在0.15～1.22 μg范围线性关系良好(r=0.999 6),加样回收率为98.5%,RSD=1.8%(n=6);5种不同制备方法 所得供试液中柚皮苷的含量分别是1.19%、1.11%、1.29%、1.18%、1.15%.结论 该法操作简单,结果 更准确,重复性好,可用于柚皮苷的含量测定.

Objective To establish a method of sample preparation for the content determination of naringin in Rhizoma Drynaria.Method The sample solution was prepared with the methods of reflux ex-tracted for 3 h and 6 h,weighing reflux extracted for 3 h,and sohxlet extracted for 3 h and 6 h.The content was determined with HPLC.stationary phase was Hypersil C18 column,the mobile phase was the methanol-acetic acid-H2O（40∶3∶60）,the flow rate was 1.0 mL/min,the column temperature was 25 ℃ and the detection wave was 283 nm.Results The linear range of nar...