期刊论文

Effect of Huanshaodan on lipofuscin content and structure stability of DNA in aging mice

中文

湖南中医药大学学报

1674-070X

2011

31

3

33-35

10.3969/j.issn.1674-070X.2011.03.009

湖南省教育厅资助项目（07C479）；

本校为第一机构

目的 研究还少丹对老龄小鼠脂褐素含量和DNA分子结构稳定性的影响.方法 将36只昆明种老年小鼠(20月龄),按体质量随机分为3组,另设12只2月龄同种小鼠作为青年对照组,共4组:①老年对照组;②老年还少丹组;③老年维生素E(VE)组;④青年对照组.连续喂养10周后,采用荧光分光光度法测定脑组织的脂褐素水平和肝组织DNA双链解旋刺余率;用紫外分光光度法测定肝组织DNA碱变性增色效应.结果 青年对照组、老年还少丹组、老年VE组的脂褐素含量明显低于老年对照组,差异有统计学意义(P

Objective To study the effect of Huanshaodan on lipofuscin content and structure stability of DNA in aging mice. Methods 36 aging Kunming mice （20months） and 12 young Kunming mice（2months） were randomly divided into four groups as following, aging control group, aging Huanshaodan group, aging vitamin E group and young control group. After feeding 10 weeks, the content of lipofuscin in brain tissue and the surplus rate of double-stranded DNA in liver tissue were detected with fluorescence spectrometry methods and spectrometry DNA alkali dege...