期刊论文

Effect of Huanshaodan on mitochondrial DNA induced deletion of myocardial by D-gal in aged mice

中文

湖南中医药大学学报

1674-070X

2011

31

5

20-22

10.3969/j.issn.1674-070X.2011.05.006.020.03

[基金项目]湖南省教育厅资助项目（07C479）.

本校为第一机构

目的 探讨还少丹对D-半乳糖(D-gal)诱导的衰老小鼠心肌线粒体DNA(mtDNA)缺失的影响.方法 将40只小鼠随机分为空白对照组、衰老模型组、还少丹低、高剂量组,用D-半乳糖建立衰老模型,分别给予蒸馏水和还少丹水煎液灌胃.连续给药6周后,采用聚合酶链反应(PCR)技术扩增心肌组织mtDNA,经琼脂糖凝胶电泳检测扩增条带,用光密度扫描定量各组mtDNA的缺失率.结果 空白对照组未见mtDNA缺失型扩增条带,其余各组均出现不同程度的缺失型扩增条带;与模型组比较,低、高剂量组小鼠心肌mtDNA缺失率明显降低,差异均具有统计学意义(P＜0.05);而低剂量与高剂量纽之间比较mtDNA的缺失率差异无统计学意义(P＞0.05).结论 ...

Objective To investigate the effect of Huanshaodan on deletion of myocardial mitochondrial DNA induced by D-gal in aged mice. Methods Forty mice were divided randomly into blank control group, aged model group, low dose group and high dose group. The models were established by injecting D-gal and feed with distilled water and Huanshaodan decoction respectively 6 weeks. The polymerase chain reaction（PCR） was used to amplified the myocardial mitochondrial DNA. The agarose gel electrophoresis and light density scanning were used respectively to ...