Objective To compare the effects of of tonifying kidney,strengthening spleen and tonifying spleen-kidney on peripheral blood T cells in chronic hepatitis patients.Methods The peripheral blood anticoagulant from 24 patients with chronic hepatitis and 10 healthy people were separated for peripheral blood mononuclear cells(PBMCs),further,T cells were extracted,separted and cultured,then,the T cells were interfered by plasma with the three groups of Chinese medicines.The expression of CD3+,CD4+,CD8+ and CD28+ in T cells were detected.Results ...