期刊论文

中文

湖南中医药大学学报

1674-070X

2012

32

7

50-53

10.3969/j.issn.1674-070X.2012.07.014.050.04

本校为第一机构

目的 建立HPLC同时测定菟丝子不同炮制品中绿原酸、金丝桃苷、槲皮素、山奈酚和异鼠李素的含量.方法 色谱条件:Kromasil ODS-1色谱柱(250 mm×4.6 mm,5μm),甲醇-0.4％磷酸水溶液为流动相,梯度洗脱,流速为1 mL/min,柱温为30℃,检测波长为360 nm.结果 5种活性成分均达到基线分离,线性关系良好(r≥0.999 7)；平均加样回收率为98.4％～100.7％,RSD≤2.4％(n＝6).结论 该方法灵敏、可靠,可用于菟丝子饮片的质量评价.

Objective To establish the method for quantitative analysis of chlorogenic acid, hyperoside, quercetin, kaempferol and isorhamnetin in the Cuscuta chinensis different pro- cessed products by HPLC. Methods The Kromasil C18 column （250 ram×4.6 mm,5 μm） was used with methanol-0.4% phosphoric acid as mobile phase. Under the condition of gradient elution, the flow rate was 1.0 mL/min, the column temperature was 30℃ and the detection wavelength was 360 nm. Results Baseline separation was obtained separately for the five kinds of active ingredien...