期刊论文

中文

湖南中医药大学学报

1674-070X

2012

32

9

15-20

10.3969/j.issn.1674-070X.2012.09.004.015.06

欧盟FP7项目（230232）； 北京市重点实验室2011年阶梯计划项目（BJ2012001）；

本校为第一机构

目的 探讨Zeylenone体外抗急性淋巴细胞白血病(Acute Lymphoblastie Leukemia,ALL)效应及作用的可能机制.方法 应用MTT法比较Zeylenone对肿瘤细胞、正常细胞增殖的影响；AO/EB染色观察其对ALL细胞(Reh、RS4;11)凋亡形态学的改变；流式细胞仪检测药物对细胞凋亡及细胞周期的影响.结果 Zeylenone对多种细胞呈现增殖抑制作用,且对ALL细胞株呈现更高的敏感性,而对外周血单个核细胞(Peripheral blood mononuclear cell,PBMC)抑制作用较小,抑制Reh、RS4; 11细胞增殖呈时间依赖性和剂量依赖性；Zeylenone能够诱导ALL细胞凋亡,阻滞细胞周期于G0/G1期.结论 Zeylenone体外具有抗ALL细胞增殖的作用,其作用与...

Objective To tial mechanism. Methods The explore the anti-ALL effects effects of Zeylenone on the of Zeylenone and its potenproliferation of cancer cells and normal cells were tested by MTT assay. The morphological changes were observed by AO/EB staining. Apoptosis and cell cycle of cells were determined by flow cytometry. Results Zeylenone inhibited the proliferation of many cell lines but had little toxicity to peripheral blood mononuclear cells（PBMC）. ALL cell lines（Reh, RS4;11） showed relative sensitive to Zeylenone. The suppressive eff...