期刊论文

Determination of the water-soluble total sugars in Malussieboldii

中文

湖南中医药大学学报

1674-070X

2013

33

5

62-64

10.3969/j.issn.1674-070X.2013.05.017.062.03

湖南中医药科技局资助项目（201161） 湖南省科学技术厅科研条件创新专项一般项目（2012TT2012） 湖南省中药学重点学科资助（湘教发[2011]76号）

本校为第一机构

目的测定三叶海棠叶中的水溶性总糖含量。方法三叶海棠叶的水提液经酸水解-乙酸乙酯萃取后,采用试样空白的蒽酮-硫酸比色法,以葡萄糖为对照品进行总糖含量测定。结果葡萄糖在7～35μg/mL范围内,吸光度与浓度的线性关系良好（r=0.998 1,n=6）,平均回收率为94.1%,RSD=1.0%（n=6）;3批三叶海棠叶中的水溶性总糖平均含量为13.1%。结论利用酸水解-乙酸乙酯萃取的差示光谱法（△A法）消除了试样基体有色和脂溶性物质的干扰,使显色后的吸收峰明显改善,结果可靠,重复性好;为三叶海棠叶中水溶性总糖的含量测定提供了一种可行的方法。

Objective To determine the water-soluble total sugars in water extract from Malussieboldii.Methods The total sugars in water extract of Malussieboldii after acid hydrolysis and extracted with ethyl acetate were determined with the anthrone-sulfuric colorimetry in which the glucose was used as standard reference.Results The concentration of glucose showed a good linearity with absorbance in the range of 7～35 μg/mL at 620 nm（r=0.998 1,n=6）.The average water soluble total sugars of three shipments of Malussieboldii was 13.1%,the average recove...