期刊论文

Deproteinization of polysaccharide from Galla chinensis

中文

湖南中医药大学学报

1674-070X

2013

33

3

44-46

10.3969/j.issn.1674-070X.2013.03.012.044.03

湖南省教育厅课题资助（10C1039）； 湖南省高校“中药新药创制与资源综合持续利用”科技创新团队（湘教通[2010]212号）； 国家中医药管理局“药用植物学”重点学科（国中医药发[2009]30号）；

本校为第一机构

目的对五倍子粗多糖进行脱蛋白处理,筛选出最佳的脱蛋白方法。方法以蛋白脱除率和多糖损失率为评价指标,选用Sevag法、酶解法以及两者联合法对五倍子粗多糖进行脱蛋白处理。结果 Sevag法、酶法以及两法联合的蛋白脱除率分别为80.8%,74.1%和82.1%,多糖损失率分别为36.1%,17.4%和21.2%。结论酶法与Sevag法联合除蛋白是去除五倍子粗多糖蛋白质的较理想方法,并优化得到了此法的最佳适用条件。

Objective To screen the optimal technology for deproteinization of polysaccharide from Galla chinensis. Methods The protein was removed by Sevag, enzyme and their combining appli- cation with the percentage of the removed protein（the PRP）and the decomposed polysaccharide（the PDP） as indexes. Results The PRPs for Scrag, enzyme and their combining application were 80.8%, 74.1% and 82.1%, the PDPs were 36.1%, 17.4% and 21.2% respectively. Conclusion The deproteinization of Sevag combined with enzyme method is an effective and al...