期刊论文

中文

湖南中医药大学学报

1674-070X

2013

33

7

41-44

10.3969/j.issn.1674-070X.2013.07.010.041.04

24306:湖南省卫生厅中医药科研项目 201210:湖南省中医药研究院科研项目

本校为第一机构

目的 测定不同药用部位、不同产地及不同采收期显脉旋覆花药材中麝香草酚和异丁酸百里香酯的含量,为该药材的质量控制与开发利用提供依据.方法 采用高效液相色谱法测定,以95%乙醇回流提取.色谱柱C18(Col-umn 200 mm×4.6 mm,5 μm),流动相∶乙腈-水(65∶35),检测波长:220 nm.结果麝香草酚和异丁酸百里香酯主要存在于显脉旋覆花的根中;麝香草酚和异丁酸百里香酯含量以湖南省通道侗族自治县较高;显脉旋覆花的采收期以9、10月份为宜.结论本研究可为显脉旋覆花药材的质量控制与开发利用提供参考.

Objective To provide a basis for quality control and development and utilization,the content determination of thymol and thymyl isobutyrate of Inula nervosa Wall from different parts,different habitats and different harvest periods were carried.Methods Inula nervosa Wall was extracted with 95% ethanol and tested by HPLC.Column C18（Column 200 mm×4.6 mm,5 μm） was used with the mobile phase of mixture of acetonitrile and water（65：35）,the flow rate was 1.0 mL/min,the detection wavelength was 220 nm,the column temperature was 25 ℃.Results Th...