期刊论文

中文

湖南中医药大学学报

1674-070X

2013

33

1

124-127

10.3969/j.issn.1674-070X.2013.01.034.0124.04

本校为第一机构

目的 比较健脾法、补肾法与解毒法对慢性乙肝病毒(HBV)感染患者外周血树突状细胞DCs介导的T淋巴细胞的影响.方法 分别收集10例HBV慢性感染免疫耐受期、免疫清除期患者和8例健康人的抗凝外周血,分离外周血单个核细胞(PBMCs),体外培养DCs,用大鼠健脾、补肾、解毒药物血浆进行干预,并建立DCs、T细胞相互作用的模型,检测T细胞的表面分子CD8+CD28+、CD8+PD-1受体的表达.结果 经补肾药物血浆(六味地黄丸)、健脾药物血浆(四君子汤)、解毒药物血浆(甘露消毒丹)处理,HBcAg负载的DCs孵育的T淋巴细胞,CD8+CD28+表达率升高,CD8+PD-1受体表达率降低.结论 补肾、健脾、解毒法在一定程度上都能通过恢复慢性HBV感...

[Abstract~ Objective To compare the effects of TCM treatment methods including Jianpi （Si- junzi decoction）, Bushen （Liuwei Dihuang decoction） and Jiedu （Ganlu Xiaodu decoction） on T lym- phocytes mediated peripheral blood DCs from persons with chronic hepatitis B virus（HBV） infection. Methods 10 patients in immune tolerance stage and 10 patients in immune clearance stage with chronic HBV infection and 8 healthy persons were included in this study. DCs were differentiated from PBMCs which were isolated from persons＇ peripheral blood, i...