期刊论文

Determination of anemonin and protoanemonin in different parts of Ranunclus ternatus Thunb by HPLC

中文

湖南中医药大学学报

1674-070X

2013

33

9

55-57+72

10.3969/j.issn.1674-070X.2013.09.009.055.03

湖南省中医药管理局科技项目资助； 省属科研究机构技术创新发展专项（2012TF1005）；

本校为第一机构

目的 建立猫爪草中的白头翁素及原白头翁素含量测定方法,分析猫爪草不同部位的白头翁素及原白头翁素.方法 采用HPLC,色谱柱为ODS C18柱(200 mm×4.6 mm,2.5μm);流动相为甲醇-水(25∶75);检测波长:白头翁素220 nm,原白头翁素260 nm;体积流量0.8 mL/min,柱温:34℃.结果 白头翁素在7.6～486.4 ng(r=0.999 8),原白头翁素在45～714 ng(r=0.999 6)有较好的线性关系;平均回收率分别为95.6％、96.8％.结论 猫爪草的不同药用部位,含量差别很大,猫爪草鲜茎叶中含原白头翁素、白头翁素,而干燥块根中未检出这两种成分.

Objective To establish the HPLC method for the determination of anemonin and protoanemon in different parts of Ranunclus ternatus ThuMb. Methods ODS C18 column （200 mm× 4.6 mm, 2.5 μm） and methanol-water （25：75.v/v） as mobile phase; the flow rate was 0.8 mL/min; detection wavelength of anemonin was 220 nm and protoanemonin was 260 nm; column temperature was 34 ℃. Results Under the chromatographic conditions, anemonin showed a good lin- ear relationship at the range of 7.6-486.4 ng （r=0.999 8） and protoanemon at the range of 45- 714 ng...