期刊论文、会议论文

Effects of plasma containing with Bushen Jiedu decoction and Jianpi Jiedu decoction on the function of peripheral blood T cells from HBV infection patients in different immunity

中文

湖南中医药大学学报

1674-070X

2013

33

1

288-295

2012年朱文峰学术思想研讨会暨中医诊断师资班30周年纪念大会

2012年朱文峰学术思想研讨会暨中医诊断师资班30周年纪念大会论文集

2012-10-01

长沙

中国中西医结合学会

中华中医药学会

10.3969/j.issn.1674-070X.2013.01.030.0108.05

国家自然科学基金资助项目(No.81072779);湖南省自然科学基金项目(No.09JJ6042)

本校为第一机构

目的:比较补肾解毒方和健脾解毒方对慢性乙肝病毒（HBV）感染不同免疫状态患者外周血树突状细胞（DCs）功能的影响。方法:选择36例湖南中医药大学第一附属医院2010年4月-2011年1月门诊慢性HBV感染患者,根据患者免疫状态分为免疫耐受组（18例）、免疫清除组（18例）,另选择10名健康人作健康组。分别采集抗凝外周静脉血,分离外周血单个核细胞（PBMCs）,进一步分离提取培养DCs,采用大鼠补肾解毒方和健脾解毒方含药血浆进行干预。对DCs进行形态学鉴定,并检测DCs表型CD1a、CD80、CD86、HLA-DR表达,观察DCs培养上清液干扰素-α（interferon-α,IFN-α）的水平。结果:免疫清除组患者干预前CD80低于健康...

　　0bjective:To investigate the effect ofTCM decoctions(BushenjieduTang and Jianpijiedu Tang)on DCs in patients with chronic hepatitis B virus infection in different immune status.Methods:36 cases of the First Affiliated Hospital of Hunan University of Traditional Chinese Medicine firom April 2010 to January 2011out-patient with chronic HBV infection, the immune status into hnmune tolerance group (18 cases), immune clearance (18 cases), and the other select 10 healthy persons as control group..DCs subset populations were diferentiated from per...