期刊论文

Effects of Mieyou Decoction on TLR2 and NF-κBp65 in Gastric Mucosa Tissues of Mice with Helicobacter pylori-related Gastritis

中文

湖南中医药大学学报

1674-070X

2014

34

7

18-22+65

10.3969/j.issn.1674-070X.2014.07.004.018.05

12JJ2046:湖南省自然科学基金 2011SK3096:湖南省科技厅科学研究项目 2013KP0111:湖南省科普计划 :湖南中医药大学研究生校级科研项目

本校为第一机构

目的 探讨灭幽汤对幽门螺杆菌相关性胃炎(HAG)小鼠胃黏膜组织Toll样受体2(TLR2)、核转因子p65(NF-κBp65)的影响.方法 将BALB/c小鼠随机分为正常对照组、模型组、胃三联组、灭幽汤组.采用复合因素(肥甘食物+湿热环境+幽门螺杆菌)建立BALB/c小鼠HP相关性胃炎脾胃湿热证模型.采用免疫组化法检测胃黏膜组织TLR2、NF-κBp65的蛋白表达情况.结果 小鼠模型组与正常对照组比较TLR2与NF-κBp65表达均增加(P＜0.05);与模型组比较,灭幽汤组小鼠胃黏膜TLR2与NF-κBp65表达均降低(P＜0.05).结论 灭幽汤能根除HP,可减轻HP引起的胃黏膜炎症反应.其机制可能是通过下调TLR2,抑制NF-κBp65的激活,从而阻止NF-κB的...

Objective To explore effects and action mechanism of Mieyou Decoction on TLR2 and NF-K BP65 in gastric mucosa tissues of mice with Helicobacter pylori-related gastritis Methods BALB/c mice were randomly assigned to normal control group, model group, gastric triad group, Mieyou group, developed a model of HAG BALB/c mice Spleen-stomach Damp-heat Syndrome by composite factors （high sugar and fat food ＋ hot and humid environment ＋ H. Pylori）. The immunohistochemical assay was adopted to detect the protein expressions of nuclear transcription f...