期刊论文

中文

湖南中医药大学学报

1674-070X

2014

34

3

28-30+36

10.3969/j.issn.1674-070X.2014.03.008.028.03

长沙市科技局科技攻关资金专项资助（K1203019-31）； 湖南省属科研机构技术创新发展专项资助（2012TF1005）；

本校为第一机构

目的 建立山银花药材中的常春藤皂苷元含量的测定方法.方法 以盐酸-乙醇溶液为水解溶剂,山银花样品加热回流4h,再用三氯甲烷萃取;采用HPLC,以Waters Symmetry C18(150 mm×4.6 mm,5μm)色谱柱,甲醇-0.4％冰醋酸水溶液(80:20)为流动相,流速为1.0 mL/min,柱温为30℃,检测波长为210 nm,测定常春藤皂苷元含量.结果 山银花中常春藤皂苷元的线性范围为2～12 μg,平均加样回收率99.0％,RSD为1.9％.不同产地山银花的常春藤皂苷元含量差异较大,湖南隆回野生的含量高达2.8％,而云南、贵州产的未检出.结论 所建立方法操作简便、准确、可靠、重复性好,可用于山银花药材中常春藤皂苷元的含量测定.

Objective To establish a new method for the determination of hederagenin in Flos Lonicerae Confusae. Methods The herb was hydrolyzed with hydrochloric acid-ethanol for 4 hours at 80 ℃ and the hederagenin was extracted with chloroform and analyzed by HPLC. The Waters Symmetry-C18 （150 mm× 4.6 mm,5 μm）was used with the mobile phase of methanol-0. 4% glacial acetic acid （80：20）, the flow rate was 1.0 mL/min, the column temperature was 30 ℃, the detected wavelength was 210 nm. Results The linear range of hederagenin was 2-12μg, the recove...