期刊论文

Effects of Dachengqi Decoction on serum acetylcholine and nitric oxide and ileocecum morphology in rats with incomplete intestinal obstruction

中文

湖南中医药大学学报

1674-070X

2014

34

3

12-16

10.3969/j.issn.1674-070X.2014.03.004.012.05

湖南省自然基金资助项目（09JJ6040）；

本校为第一机构

目的 研究大承气汤对不完全性肠梗阻大鼠血清中乙酰胆碱(Ach)和一氧化氮(NO)含量及大鼠回盲部形态学变化的影响.方法 将60只SD大鼠随机分为正常组,模型组,假手术组,大承气汤高、中、低剂量组(以下简称高、中、低剂量组),每组10只.除正常组外,假手术组用针线穿透肠系膜而不结扎,其余各组均造成不完全性肠梗阻模型.各组大鼠在手术造模前0.5 h及造模后连续给药3d.正常组、假手术组、模型组给予生理盐水,高、中、低剂量组分别给予相应剂量的大承气汤灌胃.运用酶联免疫法(ELISA)检测大鼠血清中Ach、NO含量;对大鼠回盲部进行切片、HE染色并观察其形态学变化.结果 (1)高、中、低剂量组与模型组大鼠回盲...

Objective To study the effects of Dachengqi Decoction on serum acetylcholine（Ach） and nitric oxide （NO） and ileocecum morphology in rats with incomplete intestinal obstruction. Methods 60 SD rats were randomly divided into normal group, model group, sham operation group, Dachengqi Decoction high, medium and low dose group with 10 rats in each group. Except normal group without any treatment and the sham operation group treated with needle penetration mesenteric without ligation, the other groups were prepared for incomplete intestinal obstr...