期刊论文

中文

湖南中医药大学学报

1674-070X

2014

34

4

41-45

10.3969/j.issn.1674-070X.2014.04.012.041.05

湖南省教育厅重点科研课题（11A084） 湖南省科学技术厅科技计划一般项目（2012SK3132）.

本校为第一机构

目的 探讨穴位埋药线对难治性癫痫大鼠大脑海马及皮质中多药耐药基因MDR1b的影响.方法 选用雄性SD大鼠120只,随机分为空白对照组、模型组、拉莫三嗪组、普通线组、药线组.普通线组与药线组均取大椎、肝俞(双)、血海(双)、丰隆(双)穴,先埋一侧穴位,间隔15 d后埋对侧穴位;拉莫三嗪组按照人用拉莫三嗪剂量为5 mg/(kg·d)换算灌胃大鼠,每日2次;模型组给予灌胃同等体积(2 mL/d)的蒸馏水,均干预30 d.采用荧光实时定量聚合敏链反应(RT-PCR)的方法检测多药耐药基因MDR1b在海马与颞叶皮层的表达.结果 与模型组相比,药线、普通线、拉莫三嗪干预后能降低致痫大鼠海马区多药耐药基因MDR1b的表达水平(P＜0.05...

Objective To investigate the effects of acupuncture therapy on buried intractable epilepsy hippocampus and cerebral cortex of multidrug resistance gene MDR1b. Method Hip- pocampal dosing can be located and was injected kainic acid to induce epilepsy modelby microsyringe according to Paxions Watras map and under the guidance of brain ster-eotaxic apparatus. The rats whose seizure behavior level in Racines standard V level or above were intragastric injection administration with valproate and carbamazepine intervention for 14 days, Sub-convulsion...