期刊论文

Effects of Zhibai Dihuang Decoction on Spermatogenic Cell Mitochondrial Succinate Dehydrogenase of Rats Infected by Ureaplasma Urealyticum

中文

湖南中医药大学学报

1674-070X

2014

34

11

1-4

10.3969/j.issn.1674-070X.2014.11.001.001.04

81373641:国家自然科学基金 2011sk3104:湖南省科技厅项目 K1301014-31:长沙市科技局项目 2012SK31387:湖南省科技计划

本校为第一机构

目的 探讨知柏地黄汤对解脲支原体(ureaplasma urealyticum,UU)感染大鼠生精细胞线粒体琥珀酸脱氢酶(SDH)的影响.方法 将40只SD雄性大鼠,随机分成正常组和造模组,采用经膀胱注射UU标准株悬液接种,建立UU感染动物模型,再将造模成功的大鼠随机分为模型组、阿奇霉素组(西药组)、知柏地黄汤组(中药组).采用彩色精子动态检测系统检测精子运动参数;ELISA方法检测SDH的活性及含量;RT-PCR法检测SDH mRNA表达.结果 与正常组比较,模型组大鼠精子活力、SDH活性和含量以及SDH mRNA表达均明显降低(P＜0.01).与模型组比较,中药组和西药组精子活力及SDH含量均提高(P＜0.05,P＜0.01),中药组SDH活性提高(P＜0.01)...

Objective This study was to explore the effects of Zhibai Dihuang Decoction on spermatogenic cell mitochondrial succinate dehydrogenase of rats infected by ureaplasma urealyticum （UU）. Methods The 40 SD rats were randomly divided into normal control group and model group, their bladders were inoculated with UU suspension to establish the UU infection models. Then successful model rats were randomly divided into model group, the azithromycin group （western medicine group）, and Zhibai Dihuang Decoction group （TCM group）. Sperm motility para...