期刊论文

Effects of Yuxianling Decoction on the Expression of Multiple Drug Resistant Gene MDR1b in Hippocampusand Temporal Lobe Cortex of Epileptic Rats Induced by Kainic Acid

中文

湖南中医药大学学报

1674-070X

2015

11

18-21

10.3969/j.issn.1674-070X.2015.11.005

湖南省科学技术厅科技计划一般项目（2012SK3132）； 湖南省教育厅重点科研课题（11A084）；

本校为第一机构

目的探讨愈痫灵方对耐药性癫痫模型大鼠海马及颞叶皮质多药耐药基因MDR1b表达的影响。方法 1造模:在脑立体定位仪引导下,海马区微量进样器注入红藻氨酸（KA）1.0μL（浓度为1.0μg/μL）点燃造模,选取发作行为级别在Racines标准Ⅳ级以上者,以丙戊酸钠及卡马西平灌胃干预14 d,再次亚惊厥剂量0.5μL KA复燃,选择再次发作级别在Ⅳ级以上或持续状态大鼠,且脑电图检测有癫痫样波发放者筛选为造模成功的耐药难治性癫痫模型鼠。2分组与处理:造模成功鼠随机分为愈痫灵干预组、拉莫三嗪对照组、模型组,另设假手术对照组、空白对照组共计5组,每组12只。分别给予愈痫灵汤剂、拉莫三嗪及同等体积的蒸馏水（2...

〔Abstract〕 Objective To investigate the effects of Yuxianling Decoction (YXLD) on expression of multiple drug resistant gene MDR1b in hippocampus and temporal lobe cortex of epileptic rats induced by kaicic acid (KA). Methods (1) Making models:The Hippocampus in rats located by brain stereotactic apparatus was microinjected 1μg KA (1.0 μg/μL) to kindle seizures models. The rats at above seizure behavior surpass Ⅳ level were intragastric administration interfered by sodium valproate and carbamazepine for 14 days, and rekindled 0.5 μL. The...