期刊论文

Simultaneous Determination of Four Triterpene Sapoulns from the leaves of Acanthopanax gracilistylus by HPLC

中文

湖南中医药大学学报

1674-070X

2015

35

1

28-31

10.3969/j.issn.1674-070X.2015.01.008.028.04

湖南省自然科学基金资助项目（11JJ2042）；

本校为第一机构

目的建立同时测定细柱五加叶总苷元提取物中4种三萜苷元的高效液相色谱（HPLC）分析方法。方法采用Phenomenex Columbus C18110A色谱柱（250 mm×4.60 mm,5.0μm）;柱温30℃;以乙腈-0.2%磷酸水为流动相,梯度洗脱;检测波长210 nm;流速1 m L/min。结果 4个成分均能达到基线分离,样品含量测定出各组分在相应的浓度范围内均呈现良好线性关系,其平均加样回收率分别为97.8%,96.0%,96.4%,98.4%。结论本方法可用于同时测定细柱五加叶总苷元提取物中3α,11α,23-trihydroxy-lup-20（29）-en-28-oic acid、3α,11-dihydroxy-23-oxo-lup-20（29）-en-28-oic acid、impressic acid、acankoreanogein的含量,为...

Objective To establish HPLC methods for the simultaneous determination of four triterpene saponins from the leaves of Acanthopanax gracilistylus. Methods The HPLC method was established. A Phenomenex Columbus C18 ll0A column （250 mm ×4.60 mm, 5.0 μm） was used for separation at temperature 30 ℃. The samples were gradient eluted with acetonitrile -0.1% phosphoric acid. The detection wavelength was at 210 nm and the flow rate was at 1 mLtmin. Results Four constituents in the corresponding concentration range showed a good linear relationship....