期刊论文

Effects of Zuogui Jiangtang Yishen Decoction Plasma on Caspase-12 Expression and Podocyte Cells Apoptosis in Mice Cultured in High Glucose Condition

中文

湖南中医药大学学报

1674-070X

2015

35

3

1-4+18+72

10.3969/j.issn.1674-070X.2015.03.001.001.04

81273753:国家自然科学基金 12JJ2048:湖南省自然科学基金 12JJ9031:湖南省自然科学基金 14K069:湖南省高等学校创新平台开放基金 CX2013B321:湖南省研究生科研创新项目

本校为第一机构

目的探讨在高糖作用下,体外培养的小鼠足细胞凋亡及内质网应激凋亡因子Caspase-12 m RNA和蛋白表达水平的变化及左归降糖益肾方含药血浆的调控作用。方法将体外培养的小鼠足细胞随机分为空白组（A组,25 mmol/L葡萄糖,10%空白血浆）、高糖组（B组,200 mmol/L葡萄糖,10%空白血浆）、左归降糖益肾方含药血浆组（C组,200 mmol/L葡萄糖,10%含药血浆）,4-苯基丁酸含药血浆组（D组,200 mmol/L葡萄糖,10%含药血浆）。培养48 h后,采用免疫荧光细胞化学法、流式细胞术检测足细胞凋亡情况;Western blot法、RT-PCR法分别检测Caspase-12蛋白或m RNA表达水平的变化。结果与A组比较:在高糖状态下B组足细胞凋亡率...

Objective To investigate the level of protein and m RNA expressions of Caspase-12 and cell apoptosis in podocyte cells cultured in high glucose condition in vitro, and the effects of Zuogui Jiangtang Yishen decoction plasma on Caspase-12 expression in podocyte cells. Methods The cultured podocyte cells were divided into the control group（group A,25 mmo L/L glucose, 10% blank plasma）, high glucose group（group B, 200 mmo L/L glucose, 200 mmo L/L glucose, 10% blank plasma）, Zuogui Jiangtang Yishen decoction plasma group（group C, 200 mmo L/L g...