期刊论文

Effects of Liuwei Dihuang Decoction onthe Expression of α-SMA and FN in IgA Nephropathy Rats

中文

湖南中医药大学学报

1674-070X

2015

11

22-26

10.3969/j.issn.1674-070X.2015.11.006

湖南省科技厅项目基金（2013SK3099）；

本校为第一机构

目的：观察六味地黄汤对IgA肾病大鼠α-平滑肌肌动蛋白（α-smooth muscle actin，α-SMA）及纤维连接蛋白（fibronectin， FN）表达的影响，进一步探讨其可能作用机制。方法 SD 大鼠40只随机分为正常对照组、模型组、雷公藤多苷片组，六味地黄汤组。除正常对照组外均采用牛血清白蛋白+脂多糖+四氯化碳方法建立实验性IgA肾病模型。造模成功后分组干预连续灌胃4周，分别在每周末检测尿蛋白定量及尿红细胞计数，第4周末处死各组大鼠，进行肾功能检测。采用免疫组化、Western blot检测各组α-SMA及FN蛋白表达，进行半定量分析。结果各组尿蛋白定量及尿红细胞计数比较，模型组较正常组显著升高（P＜...

〔Abstract〕 Objective To observe the effects of Liuwei Dihuang Decoction on the expression of α-SMA and fibronectin (FN) in IgA nephropathy rats, and further investigate its possible mechanism. Methods Forty SD rats were equally randomly divided into 4 groups: the normal group, model group, tripterygium glycosides tablets group and Liuwei Dihuang Decoction group. IgAN rat models were established with bovine serum albumin (BSA), lipopolysaccharide (LPS) and carbon tetrachloride (CCl4). The successfully established models were given 4 weeks of ...